Device
Part:BBa_K1919304:Design
Designed by: Shaobo Yang Group: iGEM16_SCU-China (2016-10-14)
livK
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 895
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The restriction sites are eliminated by mutation.
Source
The gene comes from genome of E.coli DH5a. We eliminated restriction site in coding region and send it to be synthsized by company.